'Dergi'
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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
JoVE Journal
Gelişim Biyolojisi
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Gelişim Biyolojisi
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
DOI:

08:01 min

February 28, 2017

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'Bölümler'

  • 00:05'Başlık'
  • 00:51Bone Marrow Cell Isolation
  • 02:45Osteoclast Differentiation
  • 03:55Jagged1-coated Surface Continuous and Temperary Notch Signaling Stimulation
  • 06:08Results: Representative Effects of Jagged1-Fc Signaling on Osteoclasts Precursor Gene Expression
  • 07:24Conclusion

Özet

'Otomatik Çeviri'

Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.

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