Here, we present a protocol to prepare and visualize secondary structures (e.g., fibers, toroidal architectures, and nano-spheres) derived from helical polycarbodiimides. The morphology characterized by both atomic force microscopy (AFM) and scanning electron microscopy (SEM) was shown to depend on molecular structure, concentration, and the solvent of choice.
Kulikov, O. V., Siriwardane, D. A., McCandless, G. T., Mahmood, S. F., Novak, B. M. Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives. J. Vis. Exp. (120), e55124, doi:10.3791/55124 (2017).