Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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January 18, 2017
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We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.
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Görlitz, F., Kelly, D. J., Warren, S. C., Alibhai, D., West, L., Kumar, S., Alexandrov, Y., Munro, I., Garcia, E., McGinty, J., Talbot, C., Serwa, R. A., Thinon, E., da Paola, V., Murray, E. J., Stuhmeier, F., Neil, M. A. A., Tate, E. W., Dunsby, C., French, P. M. W. Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (119), e55119, doi:10.3791/55119 (2017).
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