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Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
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JoVE Journal Nörobilim
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

10,405 Views

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07:13 min

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November 19, 2016

DOI:

10.3791/54801-v

DOI:
10.3791/54801-v

•

07:13 min
November 19, 2016

•

4 Views
,
1Department of Biomedical Sciences, College of Osteopathic Medicine,University of New England

Özet

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The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.

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Cite this Article

Malon, J. T., Cao, L. Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue. J. Vis. Exp. (117), e54801, doi:10.3791/54801 (2016).

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