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紫外可視およびラマン分光法イムノアッセイプラットフォームを製作
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

紫外可視およびラマン分光法イムノアッセイプラットフォームを製作

DOI:

09:02 min

November 10, 2016

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Bölümler

  • 00:05Başlık
  • 01:06Preparation of PEGylated Antibody (PEG-Ab) Solution
  • 02:25Prepare UV-Vis/Raman Probes
  • 04:57Immunoassay Plate Preparation
  • 06:08Functionalizing the Immunoassay Plate
  • 07:18Test Assay Sensitivity by UV-Vis and Raman Spectroscopy
  • 07:58Results: Determining the Sensitivity of UV-Vis and Raman Spectroscopy
  • 08:39Conclusion

Özet

Otomatik Çeviri

Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

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