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ナノスケールPCRによる動物標本における呼吸器病原体のハイスループット検出
JoVE Journal
İmmünoloji ve Enfeksiyon
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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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ナノスケールPCRによる動物標本における呼吸器病原体のハイスループット検出

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11:00 min

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November 28, 2016

DOI:

10.3791/54781-v

DOI:
10.3791/54781-v

•

11:00 min
November 28, 2016

•

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, , , , , , , , ,
1Population Medicine and Diagnostic Sciences,Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

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ナノスケールPCRによってDNA及びRNAベースの病原体のハイスループット試験は、症候性イヌおよびウマの呼吸PCRパネルを用いて説明します。

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Keyword Extraction:High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production

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Goodman, L. B., Anderson, R. R., Slater, M., Ortenberg, E., Renshaw, R. W., Chilson, B. D., Laverack, M. A., Beeby, J. S., Dubovi, E. J., Glaser, A. L. High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J. Vis. Exp. (117), e54781, doi:10.3791/54781 (2016).

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