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A Murine Model of Group B Streptococcus Vaginal Colonization
JoVE Journal
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JoVE Journal Ormancılık ve botanik
A Murine Model of Group B Streptococcus Vaginal Colonization

A Murine Model of Group B Streptococcus Vaginal Colonization

DOI:
10.3791/54708-v

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10:19 min

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November 16, 2016

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,
1Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics,University of California San Diego School of Medicine, 2Department of Biology and Center for Microbial Sciences,San Diego State University

'Bölümler'

  • 00:05'Başlık'
  • 00:46Beta-Estradiol Intraperitoneal Injection
  • 01:56Vaginal Group B Streptococcus (GBS) Inoculation
  • 04:08Vaginal Lumen Swabbing for GBS Load Quantification
  • 05:17Vaginal Lavage Fluid Collection and Tissue Dissection and Homogenization
  • 07:52Results: Representative Murine GBS Vaginal Colonization Analyses
  • 09:34Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.

'Etiketler'

Murine ModelGroup B StreptococcusVaginal ColonizationBeta-EstradiolTodd Hewitt BrothCFUInoculumGBS StrainPeritoneal CavityPBS

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