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A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

DOI:
10.3791/54620-v

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07:14 min

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October 09, 2016

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, ,
1Department of Microbiology, Tumor and Cell Biology (MTC),Karolinska Institutet

Bölümler

  • 00:05Başlık
  • 00:56Bacille Calmette-Guérin (BCG) and CFSE Injection
  • 02:08Popliteal Lymph Node (pLN) Harvest, Single Cell Suspension Preparation and Cell Surface Staining
  • 04:05Results: Migratory Skin Dendritic Cell Draining LN Relocation after BCG Footpad Infection
  • 06:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

Etiketler

CFSEDendritic CellsMigrationDraining Lymph NodesMycobacterium Bovis BCGFoot Pad InjectionCell TrackingFlow Cytometry

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