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F9를 RARE-LacZ를 셀 기반 리포터 분석을 사용하여 E8.5 배아 및 Neurosphere 문화에서 상대 레티노 산 수준의 정량적 측정
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
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F9를 RARE-LacZ를 셀 기반 리포터 분석을 사용하여 E8.5 배아 및 Neurosphere 문화에서 상대 레티노 산 수준의 정량적 측정

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09:49 min

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September 06, 2016

DOI:

10.3791/54443-v

DOI:
10.3791/54443-v

•

09:49 min
September 06, 2016

•

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1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Department of Neuroscience and Cell Biology,Rutgers University

Etiketler

Quantitative MeasurementRelative Retinoic Acid LevelsE8.5 EmbryosNeurosphere CulturesF9 RARE-LacZ Cell-based Reporter AssayRA Signaling PathwayRA Metabolic EnzymesRA ConcentrationLacZ StainingGelatin-coated PlatesCell CultureColony SelectionCell Expansion

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Ababon, M. R., Li, B. I., Matteson, P. G., Millonig, J. H. Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay. J. Vis. Exp. (115), e54443, doi:10.3791/54443 (2016).

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