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F9 RARE-lacZをセルベースレポーターアッセイを使用したE8.5胚および神経球培養における相対的なレチノイン酸レベルの定量的測定
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JoVE Journal Nörobilim
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
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F9 RARE-lacZをセルベースレポーターアッセイを使用したE8.5胚および神経球培養における相対的なレチノイン酸レベルの定量的測定

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09:49 min

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September 06, 2016

DOI:

10.3791/54443-v

DOI:
10.3791/54443-v

•

09:49 min
September 06, 2016

•

1 Views
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1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Department of Neuroscience and Cell Biology,Rutgers University

Etiketler

Quantitative MeasurementRelative Retinoic Acid LevelsE8.5 EmbryosNeurosphere CulturesF9 RARE-LacZ Cell-based Reporter AssayRA Signaling PathwayRA Metabolic EnzymesRA ConcentrationLacZ StainingGelatin-coated PlatesCell CultureColony SelectionCell Expansion

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Ababon, M. R., Li, B. I., Matteson, P. G., Millonig, J. H. Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay. J. Vis. Exp. (115), e54443, doi:10.3791/54443 (2016).

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