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فحص الإنتاجية العالية من الكربوهيدرات المهينة الانزيمات عن طريق رواية غير قابل للذوبان اللونية الركيزة الفحص أطقم
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Biyokimya
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JoVE Journal Biyokimya
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Please note that all translations are automatically generated. Click here for the English version.

فحص الإنتاجية العالية من الكربوهيدرات المهينة الانزيمات عن طريق رواية غير قابل للذوبان اللونية الركيزة الفحص أطقم

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06:51 min

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September 20, 2016

DOI:

10.3791/54286-v

DOI:
10.3791/54286-v

•

06:51 min
September 20, 2016

•

5 Views
, ,
1Department for Plant and Environmental Sciences,University of Copenhagen, 2School of Agriculture, Food and Rural Development,Newcastle University

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A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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Schückel, J., Kračun, S. K., Willats, W. G. T. High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits. J. Vis. Exp. (115), e54286, doi:10.3791/54286 (2016).

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