Journal
/
生物化学
/
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

12,588 Views

•

06:51 min

•

September 20, 2016

DOI:

10.3791/54286-v

DOI:
10.3791/54286-v

•

06:51 min
September 20, 2016

•

5 Views
, ,
1Department for Plant and Environmental Sciences,University of Copenhagen, 2School of Agriculture, Food and Rural Development,Newcastle University

Summary

Automatically generated

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

Explore More Videos

High-throughput ScreeningCarbohydrate-degrading EnzymesNovel Insoluble Chromogenic Substrate Assay KitsEnzyme DiscoveryBiomass DegradationChromogenic SubstratesEndo-cellulaseICB-wheat StrawAbsorbancePlate Reader

Read Article

Cite this Article

Schückel, J., Kračun, S. K., Willats, W. G. T. High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits. J. Vis. Exp. (115), e54286, doi:10.3791/54286 (2016).

Download .ris file

Copy

Share Video

.

Copy