Residu-specifieke Opneming van noncanonical aminozuren in Eiwitten Model behulp van een<em> Escherichia coli</em> Cell-free transcriptie-vertaalsysteem
Residu-specifieke Opneming van noncanonical aminozuren in Eiwitten Model behulp van een<em> Escherichia coli</em> Cell-free transcriptie-vertaalsysteem
An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.