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Isolamento e l'espansione delle staminali mesenchimali stromali derivate / cellule da placenta umana Tissue
JoVE Journal
Gelişim Biyolojisi
Author Produced
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
DOI:

12:40 min

June 06, 2016

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'Bölümler'

  • 00:00'Başlık'
  • 01:00Methodologies
  • 02:20Introduction
  • 03:12Tissues to be Harvested
  • 03:53Preparation of the Placenta and the Placental Anatomy
  • 04:40Dissection of the Decidua (D) Tissue
  • 05:40Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
  • 06:36Mincing and Enzymatic Digestion of the Placental Tissue
  • 07:25Collection of Mononuclear Cells and Plating into Flasks
  • 09:33Post Isolation – Cell Growth and Expansion
  • 09:57Representative Results: Culture MSC and Assay Cells for MSC Characteristics
  • 12:07Conclusion

Özet

'Otomatik Çeviri'

Qui descriviamo i metodi per la dissezione dei tessuti fetali e materni da placenta umana termine, seguito da isolamento e l'espansione delle cellule stromali / staminali mesenchimali (MSC) da questi tessuti.

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