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Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
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Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
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10:38 min
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June 11, 2016
DOI:
1Division of Hematology/Oncology,Boston Children’s Hospital, 2Harvard Tıp Fakültesi, 3Division of Hematology, Department of Medicine,Brigham and Women’s Hospital, 4Harvard Kök Hücre Enstitüsü, 5Broad Institute of Massachusetts Institute of Technology, 6Howard Hughes Medical Institute, 7Division of Hematologic Malignancies,Dana-Farber Cancer Institute
Özet
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This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.
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Hagedorn, E. J., Cillis, J. L., Curley, C. R., Patch, T. C., Li, B., Blaser, B. W., Riquelme, R., Zon, L. I., Shah, D. I. Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae. J. Vis. Exp. (112), e54168, doi:10.3791/54168 (2016).
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