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Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

11,222 Views

10:38 min

June 11, 2016

DOI:

10:38 min
June 11, 2016

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Summary

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This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

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