This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.
Hagedorn, E. J., Cillis, J. L., Curley, C. R., Patch, T. C., Li, B., Blaser, B. W., Riquelme, R., Zon, L. I., Shah, D. I. Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae. J. Vis. Exp. (112), e54168, doi:10.3791/54168 (2016).