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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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March 13, 2016
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We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.
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Nanofluidic JunctionPDMS Microfluidic ChipSelf-assemblyColloidal ParticlesSilica NanoparticlesCation-selective MembraneDNA And Protein ConcentrationDouble-molding ProcessSurface FunctionalizationIon DepletionConcentration IncreaseSilica Bead SuspensionPolyelectrolytes (PAHPSS)Surface Charge Modification
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Wei, X., Syed, A., Mao, P., Han, J., Song, Y. Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles. J. Vis. Exp. (109), e54145, doi:10.3791/54145 (2016).
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