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Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
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Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
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09:31 min
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October 16, 2016
DOI:
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology,Seoul St. Mary’s Hospital, Republic of Korea, 2Division of Rheumatology, Department of Internal Medicine,Seoul St. Mary’s Hospital, Institute of Medical Science, Republic of Korea, 3College of Medicine,The Catholic University of Korea, Republic of Korea
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Here we describe a protocol for generating human induced-pluripotent stem cells from patient-derived fibroblast-like synoviocytes, using a lentiviral system without feeder cells.
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Rim, Y. A., Park, N., Nam, Y., Ju, J. H. Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients. J. Vis. Exp. (116), e54072, doi:10.3791/54072 (2016).
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