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采用轻质板材荧光显微镜到图像斑马鱼眼睛发育
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
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采用轻质板材荧光显微镜到图像斑马鱼眼睛发育

33,362 Views

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13:01 min

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April 10, 2016

DOI:

10.3791/53966-v

DOI:
10.3791/53966-v

•

13:01 min
April 10, 2016

•

23 Views
, , , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Özet

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Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Icha, J., Schmied, C., Sidhaye, J., Tomancak, P., Preibisch, S., Norden, C. Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development. J. Vis. Exp. (110), e53966, doi:10.3791/53966 (2016).

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