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단일 프로브의 응용 프로그램 : 주변 조건에서 질량 분석 이미징 및 단일 세포 분석
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

단일 프로브의 응용 프로그램 : 주변 조건에서 질량 분석 이미징 및 단일 세포 분석

DOI:

15:00 min

June 14, 2016

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Bölümler

  • 00:05Başlık
  • 01:23Mouse Tissue Section Preparation
  • 02:31Cell Culture
  • 04:25Single-probe Fabrication
  • 06:18Build the Integrated Single-probe MS Setup
  • 07:41Ambient Mass Spectrometry Imaging (MSI)
  • 10:02In-situ Live SCMS
  • 11:31Results: The Single-probe System Was Successfully Established for Mass Spectrometry Analysis of Live Single Cells and Mass Spectrometry Imaging of Biological Tissues
  • 12:47Conclusion

Özet

Otomatik Çeviri

Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.

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