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Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
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JoVE Journal Nörobilim
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

7,842 Views

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08:20 min

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March 28, 2016

DOI:

10.3791/53818-v

DOI:
10.3791/53818-v

•

08:20 min
March 28, 2016

•

1 Views
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1Department of Neuroscience, School of Medicine,Tufts University, 2Molecular Neurobiology Laboratory,The Salk Institute for Biological Studies, 3Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute,University of California, San Francisco

Özet

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Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.

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Optical ControlNeuronal ProteinGenetically EncodedUnnatural Amino AcidNeuronsHippocampal NeuronsCalcium Phosphate TransfectionNeuronal SignalingBrain FunctionSpatial Temporal Resolution

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Cite this Article

Kang, J., Kawaguchi, D., Wang, L. Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons. J. Vis. Exp. (109), e53818, doi:10.3791/53818 (2016).

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