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心外膜生长培养测定和<em>离体</em>心外膜源性细胞迁移的评估
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JoVE Journal Gelişim Biyolojisi
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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心外膜生长培养测定和<em>离体</em>心外膜源性细胞迁移的评估

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07:44 min

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March 18, 2016

DOI:

10.3791/53750-v

DOI:
10.3791/53750-v

•

07:44 min
March 18, 2016

•

2 Views
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1Aab Cardiovascular Research Institute,University of Rochester School of Medicine and Dentistry, 2Tıp Fakültesi,University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology,University of Rochester School of Medicine and Dentistry

Özet

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Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

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Trembley, M. A., Velasquez, L. S., Small, E. M. Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration. J. Vis. Exp. (109), e53750, doi:10.3791/53750 (2016).

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