Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.
Chanana, V., Tumturk, A., Kintner, D., Udho, E., Ferrazzano, P., Cengiz, P. Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia. J. Vis. Exp. (116), e53695, doi:10.3791/53695 (2016).