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Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
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JoVE Journal Gelişim Biyolojisi
Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

DOI:
10.3791/53616-v

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07:16 min

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January 07, 2016

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1Research Center for Regenerative Medicine,Fondazione IRCCS Policlinico San Matteo, 2Department of Biology and Biotechnology “L. Spallanzani”,University of Pavia

Bölümler

  • 00:05Başlık
  • 00:47Ovary Harvest
  • 02:29Antral Oocyte Isolation
  • 04:12Antral Oocyte Sorting
  • 05:28Results: Representative Oocytes Stained and Sorted with Hoechst33342
  • 05:53Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we present a protocol for the characterization of mouse antral oocytes based on nucleolar organization.

Etiketler

Mouse Antral OocytesNucleolar Chromatin OrganizationSN (surrounded Nucleolus)NSN (non-surrounded Nucleolus)Pregnant Mare Serum GonadotropinM2 MediumGlass Mouth PipettesAspirator TubeOvary IsolationOvarian FollicleGelişim Biyolojisi

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