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Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
JoVE Journal
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Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

8,717 Views

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11:19 min

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March 07, 2016

DOI:

10.3791/53555-v

DOI:
10.3791/53555-v

•

11:19 min
March 07, 2016

•

3 Views
, , , , ,
1U.S. Army Center for Environmental Health Research, 2Nanohmics, Inc.

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This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.

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Brennan, L. M., Widder, M. W., McAleer, M. K., Mayo, M. W., Greis, A. P., van der Schalie, W. H. Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity. J. Vis. Exp. (109), e53555, doi:10.3791/53555 (2016).

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