'Dergi'
/
Kimya
/
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
JoVE Journal
Kimya
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Kimya
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

DOI:
10.3791/53410-v

•

11:26 min

•

March 21, 2016

•

,
1Department of Biological Chemistry,Weizmann Institute of Science, 2Migal-Galilee Research Institute

'Bölümler'

  • 00:05'Başlık'
  • 00:49Prepare Chlorophyll a Stock Solutions
  • 04:11Prepare Organic and Aqueous Phases of Emulsion
  • 06:43Assembly of WSCP with Chlorophyll a in Emulsion
  • 09:05Results: Assembly of WSCP Apoproteins Using Water in Oil Emersions
  • 10:15Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.

'Etiketler'

Water In Oil EmulsionsChlorophyll-binding ProteinsHydrophobic PigmentsChlorophyll ExtractionSpirulina PlatensisCyanobacteriaDEAE-SepharoseAcetoneMethanolDiethyl EtherProtein-pigment Complexes

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

High-pressure Sapphire Cell for Phase Equilibria Measurements of CO2/Organic/Water Systems

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of a Water-soluble Metal–Organic Complex Array

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction

Stereolithographic 3D Printing with Renewable Acrylates

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Read Article