Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.
Bhushan, S., Aslani, F., Zhang, Z., Sebastian, T., Elsässer, H., Klug, J. Isolation of Sertoli Cells and Peritubular Cells from Rat Testes. J. Vis. Exp. (108), e53389, doi:10.3791/53389 (2016).