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인간의 기증자 눈에서 브루흐의 멤브레인의 농축
JoVE Journal
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Enrichment of Bruch’s Membrane from Human Donor Eyes
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인간의 기증자 눈에서 브루흐의 멤브레인의 농축

11,695 Views

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10:22 min

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November 15, 2015

DOI:

10.3791/53382-v

DOI:
10.3791/53382-v

•

10:22 min
November 15, 2015

•

7 Views
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1Centre for Ophthalmology & Vision Sciences, Institute of Human Development,University of Manchester, 2Centre for Advanced Discovery and Experimental Therapeutics, Central Manchester University Hospitals NHS Foundation Trust,Manchester Academic Health Science Centre

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Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

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Age-related Macular DegenerationAMDBruch’s MembraneRetinal Pigment Epithelial CellsRPEDrusenExtracellular MatrixProtein CompositionBiochemical AnalysisMacula

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McHarg, S., Brace, N., Bishop, P. N., Clark, S. J. Enrichment of Bruch's Membrane from Human Donor Eyes. J. Vis. Exp. (105), e53382, doi:10.3791/53382 (2015).

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