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Enrichment of Bruch’s Membrane from Human Donor Eyes
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Enrichment of Bruch’s Membrane from Human Donor Eyes

Enrichment of Bruch’s Membrane from Human Donor Eyes

DOI:
10.3791/53382-v

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10:22 min

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November 15, 2015

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, , ,
1Centre for Ophthalmology & Vision Sciences, Institute of Human Development,University of Manchester, 2Centre for Advanced Discovery and Experimental Therapeutics, Central Manchester University Hospitals NHS Foundation Trust,Manchester Academic Health Science Centre

Kapitel

  • 00:05Titel
  • 01:14Enrichment of Whole Bruch’s Membrane
  • 05:00Solubilization of Whole Enriched Bruch’s Membrane and Western Blot Analysis
  • 06:34Enrichment of Macular Bruch’s Membrane
  • 08:51Results: Histology and Protein Content of Bruch’s Membrane
  • 09:35Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

Tags

Keywords: Age-related Macular DegenerationAMDBruch’s MembraneRetinal Pigment Epithelial CellsRPEDrusenExtracellular MatrixProtein CompositionBiochemical AnalysisMacula

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