Dergi
/
Nörobilim
/
Enrichissement de la membrane de Bruch des yeux de donneur humain
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Enrichment of Bruch’s Membrane from Human Donor Eyes
Please note that all translations are automatically generated. Click here for the English version.

Enrichissement de la membrane de Bruch des yeux de donneur humain

11,695 Views

•

10:22 min

•

November 15, 2015

DOI:

10.3791/53382-v

DOI:
10.3791/53382-v

•

10:22 min
November 15, 2015

•

7 Views
, , ,
1Centre for Ophthalmology & Vision Sciences, Institute of Human Development,University of Manchester, 2Centre for Advanced Discovery and Experimental Therapeutics, Central Manchester University Hospitals NHS Foundation Trust,Manchester Academic Health Science Centre

Özet

Automatically generated

Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

Explore More Videos

Age-related Macular DegenerationAMDBruch’s MembraneRetinal Pigment Epithelial CellsRPEDrusenExtracellular MatrixProtein CompositionBiochemical AnalysisMacula

Read Article

Cite this Article

McHarg, S., Brace, N., Bishop, P. N., Clark, S. J. Enrichment of Bruch's Membrane from Human Donor Eyes. J. Vis. Exp. (105), e53382, doi:10.3791/53382 (2015).

Download .ris file

Copy

Share Video

.

Copy