Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
خطوة محددة الفرز ماوس Spermatids بواسطة التدفق الخلوي
10,331 Views
•
06:31 min
•
December 31, 2015
DOI:
Özet
Automatically generated
We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.
Read Article
Cite this Article
Simard, O., Leduc, F., Acteau, G., Arguin, M., Grégoire, M., Brazeau, M., Marois, I., Richter, M. V., Boissonneault, G. Step-specific Sorting of Mouse Spermatids by Flow Cytometry. J. Vis. Exp. (106), e53379, doi:10.3791/53379 (2015).
Copy