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发育生物学
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Step-specific Sorting of Mouse Spermatids by Flow Cytometry
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

DOI:
10.3791/53379-v

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06:31 min

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December 31, 2015

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, , , , , , , ,
1Department of Biochemistry,Université de Sherbrooke, 2Department of Medicine,Université de Sherbrooke

Chapters

  • 00:05Title
  • 00:50Tube and Cell Preparation
  • 03:01Cell Sorting
  • 04:50Results: Validation of the Purification Scheme by Epifluorescence Microscopy
  • 05:46Conclusion

Summary

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We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.

Tags

Keywords: Spermatid DifferentiationSpermatid SortingFlow CytometrySpermatid PopulationsChromatin RemodelingStep-specific MarkersGenomic AnalysisProteomic Analysis

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