Here we present a protocol to determine the minimum number images that needed to be registered and averaged to resolve subcortical structures and test whether the individual layers of the LGN could be resolved in the absence of physiological noise.
McKetton, L., Williams, J., Viviano, J. D., Yücel, Y. H., Gupta, N., Schneider, K. A. High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem. J. Vis. Exp. (106), e53309, doi:10.3791/53309 (2015).