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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

13,253 Views

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09:55 min

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July 04, 2016

DOI:

10.3791/53245-v

DOI:
10.3791/53245-v

•

09:55 min
July 04, 2016

•

2 Views
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1Department of Biological Sciences, Markey Center for Structural Biology,Purdue Üniversitesi, 2National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK),National Institutes of Health, 3National Institute of General Medical Sciences (NIGMS),National Institutes of Health

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β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR spectroscopy.

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Noinaj, N., Mayclin, S., Stanley, A. M., Jao, C. C., Buchanan, S. K. From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins. J. Vis. Exp. (113), e53245, doi:10.3791/53245 (2016).

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