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Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Bölümler
- 00:05Başlık
- 01:23DNA Extraction from FFPE Reference Standard Cell Lines
- 03:33Digital Droplet PCR: Droplet Generation
- 05:56Digital Droplet PCR: PCR Amplification
- 07:36Digital Droplet PCR: Droplet Reading and DNA Mutation Profiling
- 08:28Results: ddPCR Detects BRAF V600E Mutations in FFPE Reference Standard Cell Lines
- 09:20Conclusion
The goal of this video is to demonstrate how to perform automated DNA extraction from formalin-fixed paraffin-embedded (FFPE) reference standard cell lines and digital droplet PCR (ddPCR) analysis to detect rare mutations in a clinical setting. Detecting mutations in FFPE samples demonstrates the clinical utility of ddPCR in FFPE samples.
