/
/
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
9,390 Views
•
08:22 min
•
September 30, 2015
DOI:
Özet
Automatically generated
Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.
Read Article
Cite this Article
Villa-Cuesta, E., Rand, D. M. Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila. J. Vis. Exp. (103), e53149, doi:10.3791/53149 (2015).
Copy