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Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
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Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
1Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, UMR 535,Université Montpellier, 2Centre d’études d’agents Pathogènes et Biotechnologies pour la Santé, CNRS, FRE 3689,Université Montpellier, 3Unité de Formation et de Recherche des Sciences de la Santé, EA3647-EPIM,Université Versailles St Quentin
Bölümler
- 00:05Başlık
- 01:35Preparation of M. abscessus Inocula
- 03:20Microinjection of Zebrafish Embryos
- 05:25Generation of Macrophage-depleted Embryos
- 06:13Bacterial Burden Quantification
- 07:16Imaging M. abscessus-infected Embryos
- 08:34Results: M. abscessus Infection in Zebrafish Embryos
- 10:03Conclusion
Optically transparent zebrafish embryos are widely used to study and visualize in real time the interactions between pathogenic microorganisms and the innate immune cells. Micro-injection of Mycobacterium abscessus, combined with fluorescence imaging, is used to scrutinize essential pathogenic features such as cord formation in zebrafish embryos.
