Dergi
/
Biyoloji
/
Strand DNA Deslocamento Portas para redes de reação de Execução químicos derivados do plasmídeo
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Strand DNA Deslocamento Portas para redes de reação de Execução químicos derivados do plasmídeo

13,936 Views

•

07:50 min

•

November 25, 2015

DOI:

10.3791/53087-v

DOI:
10.3791/53087-v

•

07:50 min
November 25, 2015

•

2 Views
, ,
1Department of Electrical Engineering,Washington Üniversitesi, 2Department of Computer Science & Engineering,Washington Üniversitesi

Özet

Automatically generated

This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

Explore More Videos

DNA NanotechnologyPlasmid DNANicked Double-stranded DNA (ndsDNA)Strand Displacement GatesChemical Reaction NetworksMolecular ProgrammingEnzymatic ProcessingFluorescence Kinetics

Read Article

Cite this Article

Chen, Y., Rao, S. D., Seelig, G. Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks. J. Vis. Exp. (105), e53087, doi:10.3791/53087 (2015).

Download .ris file

Copy

Share Video

.

Copy