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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Biologie
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Journal JoVE Biologie
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

13,926 Views

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07:50 min

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November 25, 2015

DOI:

10.3791/53087-v

DOI:
10.3791/53087-v

•

07:50 min
November 25, 2015

•

2 Views
, ,
1Department of Electrical Engineering,University of Washington, 2Department of Computer Science & Engineering,University of Washington

Summary

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This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.

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DNA NanotechnologyPlasmid DNANicked Double-stranded DNA (ndsDNA)Strand Displacement GatesChemical Reaction NetworksMolecular ProgrammingEnzymatic ProcessingFluorescence Kinetics

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Cite this Article

Chen, Y., Rao, S. D., Seelig, G. Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks. J. Vis. Exp. (105), e53087, doi:10.3791/53087 (2015).

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