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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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August 07, 2015
DOI:
1Biyomedikal Bilimler bölümü,Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute,Seoul National University Hospital, 3College of Life Sciences,Gwangju Institute of Science and Technology, 4Department of Ophthalmology,Seoul National University College of Medicine
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Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.
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Park, S. W., Kim, J. H., Park, W. J., Kim, J. H. Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium. J. Vis. Exp. (102), e53030, doi:10.3791/53030 (2015).
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