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Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester
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July 07, 2015
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Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.
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Jebelli, J., Piers, T., Pocock, J. Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester. J. Vis. Exp. (101), e52983, doi:10.3791/52983 (2015).
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