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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

11,986 Views

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15:57 min

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July 11, 2015

DOI:

10.3791/52833-v

DOI:
10.3791/52833-v

•

15:57 min
July 11, 2015

•

2 Views
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1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine,Iowa State Üniversitesi, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service,United States Department of Agriculture

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Long-term cultured interferon-γ enzyme-linked immunospot assay is used as a measure of central memory responses and correlates with protective anti-mycobacterial vaccine responses. With this assay, peripheral blood mononuclear cells are stimulated with mycobacterial antigens and interleukin-2 for 14 days, enabling differentiation and expansion of central memory T cells.

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Interferon-γELISPOT AssayEffector T CellsMemory T CellsCentral Memory T CellsEffector Memory T CellsCattleTuberculosisVaccinationAntigen StimulationLong-term Culture

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Maggioli, M. F., Palmer, M. V., Vordermeier, H. M., Whelan, A. O., Fosse, J. M., Nonnecke, B. J., Waters, W. R. Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle. J. Vis. Exp. (101), e52833, doi:10.3791/52833 (2015).

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