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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

DOI:
10.3791/52830-v

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10:32 min

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June 05, 2015

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, , , , , , ,
1School for Mental Health and Neuroscience,Maastricht University, 2Department of Neurosciences,Institut d’Investigació Germans Trias i Pujol

'Bölümler'

  • 00:05'Başlık'
  • 01:41Staining and Sorting PBMCs for CD22+ and IgG+ Cells
  • 02:46Plating the Sorted PBMCs Including EBV Infection
  • 04:13An ELISA for IgG Antibodies
  • 06:19Amplifying, Cloning and Sequencing Heavy and Light Chain DNA
  • 07:26Producing Antibodies in HEK Cells
  • 08:20Anticipated Results
  • 09:40Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.

'Etiketler'

Recombinant Human IgGMonoclonal AntibodiesImmortalized B CellsEpstein-Barr VirusToll-like Receptor 9Peripheral Blood Mononuclear CellsAntibody CloningImmunotherapeuticsAutoimmunity

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