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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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June 16, 2015
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1Laboratory for Biomaterials and Bioengineering, Department Min-Met-Materials Eng & CHU de Québec Research Center, Canada Research Chair I for the Innovation in Surgery,Laval University, 2NSERC CREATE Program for Regenerative Medicine (NCPRM),Laval University, 3Department Electronics, Information and Bioengineering,Politecnico di Milano, 4Department of Chemical and Materials Engineering,Alberta Üniversitesi, 5National Institute for Nanotechnology,National Research Council (Canada), 6Department of Chemical and Biochemical Engineering,University of Western Ontario
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In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.
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Meghezi, S., Seifu, D. G., Bono, N., Unsworth, L., Mequanint, K., Mantovani, D. Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration. J. Vis. Exp. (100), e52812, doi:10.3791/52812 (2015).
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