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Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

DOI:
10.3791/52802-v

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07:37 min

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July 20, 2015

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, , , , ,
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 2Department of Biological Structure,University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center

'Bölümler'

  • 00:05'Başlık'
  • 01:33Head Muscle (Masseter, Digastric, and Levator Veli Palatini) Dissection
  • 04:01Satellite Cell Isolation and Culture
  • 06:18Results: Representative Myotube Generation from Rat Head Branchiometric Muscle Tissue
  • 07:09Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

'Etiketler'

Satellite CellsHead Branchiomeric MusclesFibrosisMuscle RegenerationCleft PalateIn Vitro CultureEnzymatic DigestionExtracellular Matrix Gel

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