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Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans

DOI:
10.3791/52742-v

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07:51 min

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June 24, 2015

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, ,
1Max Planck Institute for Biophysical Chemistry

'Bölümler'

  • 00:05'Başlık'
  • 01:00Instruments, Culture Media and Dishes
  • 01:58Preparation of Agarose Microchambers
  • 04:50Agarose Microfluidic Chambers Imaging
  • 05:37Calcium Imaging
  • 06:27Results: Long-term Calcium Imaging
  • 07:20Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Imaging behavior and neural activity over long time scales without immobilization of the animal is a prerequisite to understand behavior. Agarose microfluidic chambers imaging (AMI) can be used to image neural activity and behavior for all life stages of Caenorhabditis elegans.

'Etiketler'

Calcium ImagingCaenorhabditis ElegansAgarose MicrochambersLong-termDavranışNeural ActivityEMCCDParallel Imaging

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