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大人の海馬神経新生を研究するために、同じホスト種由来の抗体による免疫組織化学および複数のラベル
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JoVE Journal Nörobilim
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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大人の海馬神経新生を研究するために、同じホスト種由来の抗体による免疫組織化学および複数のラベル

26,336 Views

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09:24 min

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April 22, 2015

DOI:

10.3791/52551-v

DOI:
10.3791/52551-v

•

09:24 min
April 22, 2015

•

1 Views
, , ,
1Hans Berger Department of Neurology,Jena University Hospital

Özet

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This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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Cite this Article

Ansorg, A., Bornkessel, K., Witte, O. W., Urbach, A. Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis. J. Vis. Exp. (98), e52551, doi:10.3791/52551 (2015).

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