Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
Bölümler
- 00:05Başlık
- 02:17Injection of Thymidine Analogs and Tissue Preparation
- 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
- 06:27Imaging and Data Analysis
- 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
- 08:42Conclusion
This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.
