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Immunhistochemie und Multiple Markierung mit Antikörper aus dem gleichen Host Arten, die Adult Neurogenese Studieren
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JoVE Journal Nörobilim
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Immunhistochemie und Multiple Markierung mit Antikörper aus dem gleichen Host Arten, die Adult Neurogenese Studieren

26,336 Views

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09:24 min

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April 22, 2015

DOI:

10.3791/52551-v

DOI:
10.3791/52551-v

•

09:24 min
April 22, 2015

•

1 Views
, , ,
1Hans Berger Department of Neurology,Jena University Hospital

Özet

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This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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Ansorg, A., Bornkessel, K., Witte, O. W., Urbach, A. Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis. J. Vis. Exp. (98), e52551, doi:10.3791/52551 (2015).

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