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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Gelişim Biyolojisi
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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

21,944 Views

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10:41 min

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March 17, 2015

DOI:

10.3791/52458-v

DOI:
10.3791/52458-v

•

10:41 min
March 17, 2015

•

17 Views
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1Centre for Integrative Physiology,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh

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This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.

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Morgan, S., Campbell, L., Allison, V., Murray, A., Spears, N. Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles. J. Vis. Exp. (97), e52458, doi:10.3791/52458 (2015).

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